English: This painting of a private gallery or cabinet of a Flemish collector depicts a visit by Archdukes Albert and Isabella, the Habsburg governors of the Southern Netherlands. Isabella is seated, while her husband stands to her right and their unidentified host, behind.

The walls are covered with paintings by Flemish artists. The sculpture displayed throughout is from various schools, but includes the bronze "Allegory of Architecture" by Giambologna, a Flemish sculptor who made his fortune in Florence. A painted "Allegory of Iconoclasm," depicting people who destroy art as animals, rests against a chair. Visitors examining paintings and objects on the tables draw the viewer's attention to these objects, as well as shells and a stuffed bird of paradise, from the Spice Islands. Pets include a monkey, kept out of mischief on a chain, and a dog, apparently with two heads (an alteration by the artist that has "bled" through). The globe-like object on the table at the left is one of Cornelis Drebbels' attempts at a perpetual-motion clock; the principles which ran it are now lost.

Depictions of art collections were a specialty of Antwerp painters. Albert's and Isabella's role as rulers and patrons of the arts is celebrated here in an unprecedented way. The immense vase of flowers by Jan Brueghel, the greatest Flemish flower painter, is crowned by a large sunflower. This South American flower which could grow to be 14 feet tall and could turn toward the sun, was first seen by Europeans in the mid-1500s. It had been illustrated as a New World wonder in botanical treatises, but this is its earliest inclusion in a painting and its earliest use as a symbol of princely patronage. In turning to the sun (but here toward Albert and Isabella), it symbolizes the way that the arts grow and blossom in the light and warmth of princely patronage.